Adaptor dimer is a byproduct formed during the ligation of adaptors to both ends of the target DNA fragment. If not removed, the adaptor dimers will compete for binding to flow cell and for PCR amplification with desired target ligated to adaptors, and significantly lower the sequencing efficiency and quality
Powered by XNA technology, Dimerator™ uses XNA clamps to block adapter-adapter ligation and improves adapter dimer to tagged library ratio, which leads to significantly higher quality sequencing data. This technology has solved the problem of formed dimer when 5’ and 3’ adapters self-ligate without library insert and requires no gel purification to remove dimer. It also
Increase valuable sequencing reads
Handle low inputs and small RNA (e.g. miRNA)
Tag efficiently in samples with low target abundance
Full automation possible
Adapter Dimer Removal for NGS Library Preparation
Traditional Methods for Adaptor Dimer Removal
Traditional methods to remove adaptor dimers include, not limited to:
Magnetic Beads Binding: it takes time and performance varies depending on different vendors
Gel Purification: it is time consuming and sample loss often occurs
Adaptor: target ratio optimization: it helps but does not solve problems and may waste sample for testing.
Changing Adaptor Design to Reduce Dimer Formation: it helps but does not completely solve the problem.
How Does Dimerator™ Adapter Dimer Removal Work?
After ligating a 3´-adenylated DNA adapter to the 3´-end of miRNA in total RNA, an RNA adapter is ligated to the 5´-end of the miRNA. The ligations also produce an unwanted adapter-dimer side product as shown in the figure. The Dimerator™ works by blocking this side product from amplifying by RT-PCR. However, the doubly ligated products are unaffected by the Dimerator™ and amplified to generate sequencing libraries and introduce the barcodes. Two separate reads are required to sequence the insert miRNA and barcode, respectively, on the flow cell surface with the Illumina NGS platforms.
Product Specifications for Dimerator™ Adapter Dimer Removal Test
Product Catalog Number
500-Reaction Pack Size: DC-60-0500R
1000-Reaction Pack Size: DC-60-1000R
For research use only
500 Reactions and 1000 Reactions
Stable for 12 Months at -25°C to -15°C