Dimerator™ Adapter Dimer Removal | DiaCarta, Inc.

Adaptor dimer is a byproduct formed during the ligation of adaptors to both ends of the target DNA fragment. If not removed, the adaptor dimers will compete for binding to flow cell and for PCR amplification with desired target ligated to adaptors, and significantly lower the sequencing efficiency and quality

Powered by XNA technology, Dimerator™ uses XNA clamps to block adapter-adapter ligation and improves adapter dimer to tagged library ratio, which leads to significantly higher quality sequencing data. This technology has solved the problem of formed dimer when 5’ and 3’ adapters self-ligate without library insert and requires no gel purification to remove dimer. It also prevent the amplification of the smaller dimer side product out-competes amplification of tagged library.

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Increase valuable sequencing reads

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Handle low inputs and small RNA (e.g. miRNA)

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Tag efficiently in samples with low target abundance

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Full automation possible

Adapter Dimer Removal for NGS Library Preparation

Traditional Methods for Adaptor Dimer Removal

Traditional methods to remove adaptor dimers include, not limited to:

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Magnetic Beads Binding: it takes time and performance varies depending on different vendors

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Gel Purification: it is time consuming and sample loss often occurs

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Adaptor: target ratio optimization: it helps but does not solve problems and may waste sample for testing.

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Changing Adaptor Design to Reduce Dimer Formation: it helps but does not completely solve the problem.

Dimerator_How-it-Works2

How Does Dimerator™ Adapter Dimer Removal Work?

After ligating a 3´-adenylated DNA adapter to the 3´-end of miRNA in total RNA, an RNA adapter is ligated to the 5´-end of the miRNA. The ligations also produce an unwanted adapter-dimer side product as shown in the figure. The Dimerator™ works by blocking this side product from amplifying by RT-PCR. However, the doubly ligated products are unaffected by the Dimerator™ and amplified to generate sequencing libraries and introduce the barcodes. Two separate reads are required to sequence the insert miRNA and barcode, respectively, on the flow cell surface with the Illumina NGS platforms.

Product Specifications for Dimerator™ Adapter Dimer Removal Test 

Product Catalog Number

500-Reaction Pack Size: DC-60-0500R
1000-Reaction Pack Size: DC-60-1000R

Intended Use

For research use only 

Pack Size

500 Reactions and 1000 Reactions

Stability

Stable for 12 Months at -25°C  to -15°C

Need Help?

Call us: +1 (800) 878-6662

Email us: information@diacarta.com

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