JAK2 Mutation Detection Test | DiaCarta, Inc.

To aid in the identification of patients eligible for treatment and to monitor response to therapy, DiaCarta offers QClamp® JAK2 Mutation Detection Test in plasma sample which can lead to improved outcomes in cancer patients. The QClamp® JAK2 Mutation Detection Test is an in vitro diagnostic real-time qualitative PCR assay for the detection of somatic mutations in and near JAK2 codon 617 in Exon 14 in the JAK2 tyrosine kinase gene, using purified DNA extracted from plasma.

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Powered by XNA technology, the QClamp® JAK2 Mutation Detection Test has achieved a much higher analytical sensitivity compared to other commercial qPCR kits and other cancer gene mutation detection methods. QClamp® JAK2 Mutation Detection Test is able to detect reliably 0.1% to 0.5% mutant DNA out of wild-type DNA for targeted mutations, providing lower detection limit compared with competition due to a strict enrichment of mutant sequences while suppressing amplification of wild-type sequences.

QClamp® JAK2 Mutation Detection Test is CE/IVD-certified.

Advantages of QClamp® JAK2 Mutation Detection Test

ULTRA-SENSITIVE

Detect reliably 0.1% to 0.5% VAF mutant DNA out of wild-type DNA for targeted mutations

SAMPLE READY

Suitable for plasma samples

LOW INPUT DNA

Minimum 5ng input DNA per reaction. Less than 2 tubes of blood (10mL each) needed for cfDNA

COMPREHENSIVE COVERAGE

Covering all relevant somatic mutations of JAK2 oncogene

FAST RESULTS

Less than 4 hours of assay run time

GREAT VERSATILITY

Validated on the most common qPCR machines with minimized variability

JAK2 Mutation and Cancer

JAK2 Introduction

Janus kinase 2 (JAK2) is a non-receptor tyrosine kinase that associates with the cytoplasmic domains of multiple cytokine receptors to activate downstream targets, including STATs and JAK2 itself.
 
 

JAK2 Mutations

V617F mutation in JAK2 leads to constitutive tyrosine phosphorylation activity and constitutive activation of STAT5. This mutation is common for the following patients: >90% of patients with polycythemia vera, 50% of patients with primary myelofibrosis and 60% of patients with essential thromobocytopenia.

Streamlined Workflow for QClamp® JAK2 Mutation Detection Tests

Step 1: DNA Isolation & Quantification

Extract DNA from plasma using a commercial DNA extraction kit followed by measuring the concentration using fluorometric analysis

Step 2: set up qpcr

Mix the assay reagents, load into PCR plate, add controls and extracted DNA ~ 30-60 minutes

Step 3: Amplification parameters

Enter amplification parameters on
qPCR instrument, load PCR plate
and start the run ~ 2.5 hours

Step 4: Data analysis

Determine the presence or absence
of mutations according to the Cq
value cutoffs ~ 15 minutes

Resources

Catalog Number 

CE/IVD catalog #: DC-10-0166
Research-use-only (RUO) catalog #: DC-10-0166R

Pack Size

30 samples

Detected Codons

Codon 617

Intended Use

For In vitro diagnostic Use (CE/IVD) or for research use

Sample Type

Plasma

Input DNA

5-10ng/Reaction

Validated Instruments

Roche LightCycler® 480, Bio-Rad CFX384 and ABI QuantStudio 5

Detection Channel

FAM; HEX

Detection Chemistry

TaqMan

Turnaround Time

Less than 4 hours

Stability

Stable for 12 months at -25 ℃ to -15 ℃

Most frequent JAK2 mutations detected by QClamp® JAK2 Mutation Detection Test

ExonAmino Acid ChangeNucleotide ChangeCosmic No.
14V617>F1849G>T12600

Amplification Plot from ABI QuantStudio 5

Amplification plot showing triplicates of positive controls (mutant template and wild-type template mix) and negative controls (wild-type templates only) for JAK2 TaqMan amplification using ABI QuantStudio 5. Reference gene ACTB amplification from the positive and negative controls (total 6) is shown as well.

Amplification Plot from Bio-Rad CFX384

Amplification plot showing triplicates of positive controls (mutant template and wild-type template mix) and negative controls (wild-type templates only) for JAK2 TaqMan amplification using Bio-Rad CFX384. Reference gene ACTB amplification from the positive and negative controls (total 6) is shown as well.

Amplification Plot from Roche LightCycler® 480 (FAM)

Amplification plot showing triplicates of positive controls (mutant template and wild-type template mix) and negative controls (wild-type templates only) for JAK2 TaqMan amplification using Roche LightCycler® 480.

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Ordering Information

For products that are in stock, DiaCarta will arrange shipment in 1-3 days. For products that are on backorder, DiaCarta will arrange shipment in 3-5 weeks.

Intended Use: QClamp® JAK2 Mutation Detection Test is CE/IVD certified. For the outside USA, this product is available for diagnostic use (CE/IVD) and research use (RUO). For the USA, this product is provided for research use only (RUO) and not for diagnostic use. DiaCarta ships CE/IVD version to the outside USA and ships RUO version to the USA. Please call 1-800-246-8878 or email order@diacarta.com if you have questions or specific needs.

Shipping Condition: QClamp® JAK2 Mutation Detection Test will be shipped with dry ice. For domestic shipment, DiaCarta provides overnight delivery through FedEx Domestic Overnight Shipping Service. For international shipment, DiaCarta provides 3-7 days in transit through FedEx International Priority Shipping Service. Please contact DiaCarta if you prefer to use your own shipping carrier.

Need Help?

Call us: +1 (800) 878-6662

Email us: information@diacarta.com

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