To aid in the identification of patients eligible for treatment and to monitor response to therapy, DiaCarta offers QClamp® JAK2 Mutation Detection Test in plasma sample which can lead to improved outcomes in cancer patients. The QClamp® JAK2 Mutation Detection Test is an in vitro diagnostic real-time qualitative PCR assay for the detection of somatic mutations in and near JAK2 codon 617 in Exon 14 in the JAK2 tyrosine kinase gene, using purified DNA extracted from plasma.
Powered by XNA technology, the QClamp® JAK2 Mutation Detection Test has achieved a much higher analytical sensitivity compared to other commercial qPCR kits and other cancer gene mutation detection methods. QClamp® JAK2 Mutation Detection Test is able to detect reliably 0.1% to 0.5% mutant DNA out of wild-type DNA for targeted mutations, providing lower detection limit compared with competition due to a strict enrichment of mutant sequences while suppressing amplification of wild-type sequences.
QClamp® JAK2 Mutation Detection Test is CE/IVD-certified.
Advantages of QClamp® JAK2 Mutation Detection Test
Detect reliably 0.1% to 0.5% VAF mutant DNA out of wild-type DNA for targeted mutations
Suitable for plasma samples
LOW INPUT DNA
Minimum 5ng input DNA per reaction. Less than 2 tubes of blood (10mL each) needed for cfDNA
Covering all relevant somatic mutations of JAK2 oncogene
Less than 4 hours of assay run time
Validated on the most common qPCR machines with minimized variability
JAK2 Mutation and Cancer
Janus kinase 2 (JAK2) is a non-receptor tyrosine kinase that associates with the cytoplasmic domains of multiple cytokine receptors to activate downstream targets, including STATs and JAK2 itself.
V617F mutation in JAK2 leads to constitutive tyrosine phosphorylation activity and constitutive activation of STAT5. This mutation is common for the following patients: >90% of patients with polycythemia vera, 50% of patients with primary myelofibrosis and 60% of patients with essential thromobocytopenia.
Streamlined Workflow for QClamp® JAK2 Mutation Detection Tests
Step 1: DNA Isolation & Quantification
Extract DNA from plasma using a commercial DNA extraction kit followed by measuring the concentration using fluorometric analysis
Step 2: set up qpcr
Mix the assay reagents, load into PCR plate, add controls and extracted DNA ~ 30-60 minutes
Step 3: Amplification parameters
Enter amplification parameters on
qPCR instrument, load PCR plate
and start the run ~ 2.5 hours
Step 4: Data analysis
Determine the presence or absence
of mutations according to the Cq
value cutoffs ~ 15 minutes
CE/IVD catalog #: DC-10-0166
Research-use-only (RUO) catalog #: DC-10-0166R
For In vitro diagnostic Use (CE/IVD) or for research use
Roche LightCycler® 480, Bio-Rad CFX384 and ABI QuantStudio 5
Less than 4 hours
Stable for 12 months at -25 ℃ to -15 ℃
Most frequent JAK2 mutations detected by QClamp® JAK2 Mutation Detection Test
|Exon||Amino Acid Change||Nucleotide Change||Cosmic No.|
Amplification Plot from ABI QuantStudio 5
Amplification plot showing triplicates of positive controls (mutant template and wild-type template mix) and negative controls (wild-type templates only) for JAK2 TaqMan amplification using ABI QuantStudio 5. Reference gene ACTB amplification from the positive and negative controls (total 6) is shown as well.
Amplification Plot from Bio-Rad CFX384
Amplification plot showing triplicates of positive controls (mutant template and wild-type template mix) and negative controls (wild-type templates only) for JAK2 TaqMan amplification using Bio-Rad CFX384. Reference gene ACTB amplification from the positive and negative controls (total 6) is shown as well.
Amplification Plot from Roche LightCycler® 480 (FAM)
Amplification plot showing triplicates of positive controls (mutant template and wild-type template mix) and negative controls (wild-type templates only) for JAK2 TaqMan amplification using Roche LightCycler® 480.
For products that are in stock, DiaCarta will arrange shipment in 1-3 days. For products that are on backorder, DiaCarta will arrange shipment in 3-5 weeks.
Intended Use: QClamp® JAK2 Mutation Detection Test is CE/IVD certified. For the outside USA, this product is available for diagnostic use (CE/IVD) and research use (RUO). For the USA, this product is provided for research use only (RUO) and not for diagnostic use. DiaCarta ships CE/IVD version to the outside USA and ships RUO version to the USA. Please call 1-800-246-8878 or email email@example.com if you have questions or specific needs.
Shipping Condition: QClamp® JAK2 Mutation Detection Test will be shipped with dry ice. For domestic shipment, DiaCarta provides overnight delivery through FedEx Domestic Overnight Shipping Service. For international shipment, DiaCarta provides 3-7 days in transit through FedEx International Priority Shipping Service. Please contact DiaCarta if you prefer to use your own shipping carrier.